This application note describes how the DNA 12000 LabChip® kit can be used with the Agilent Technologies 2100 bioanalyzer to size DNA fragments generated by restriction enzyme digestion. Sizing accuracy was found to be greater than 92 % for all samples analyzed. Compari- son of the chip-based separations to those obtained using agarose gel electrophoresis indicates that the DNA 12000 assay accurately sizes fragments over a wider range than can be usually analyzed on a slab gel.
This document details the use of the Agilent 2100 Bioanalyzer and the DNA 12000 LabChip kit for sizing DNA fragments generated by restriction enzyme digestion. The note compares the chip-based separation method to traditional slab gel electrophoresis, highlighting advantages such as increased speed, higher sensitivity, and automated data analysis.
| Sizing range | 100 to 12000 base pairs |
|---|---|
| Sizing accuracy | greater than 92 % |
| Detection limit | ~ 0.05 ng/pl |
| Analysis time | less than 30 minutes |
Sizing accuracy was found to be greater than 92 % for all samples analyzed.
No, the assay is compatible with commonly used restriction digest buffers so no desalting or other sample pre-treatment is necessary.
The Agilent 2100 bioanalyzer can analyze 12 DNA samples in less than 30 minutes.