The Agilent 1100 Series micro fraction collector is a high-performance solution for processing complex samples in applications such as proteomics, metabolomics, natural product research, and library generation. This Application Note describes the field of application for the Agilent 1100 Series micro fraction collector and discusses the features and software control of this device. In addition, the main modules of the micro fraction collection system are also discussed.
The document introduces the Agilent 1100 Series micro fraction collector as a high-performance solution designed to reduce sample complexity for proteomics, metabolomics, and natural product research. It is specifically developed for use with the Agilent 1100 Series capillary LC system to handle limited sample amounts effectively.
The note details the equipment comprising the collection system, including the capillary pump, autosampler, diode-array detector, column compartment, and the micro fraction collector itself controlled by ChemStation software. It explains unique features such as liquid contact control for bubble-free collection and discusses strategies for time-based or peak-based fractionation.
The collection time for each fraction can be set to a minimum of 3 seconds.
Yes, the instrument allows pooling of fractions from multiple runs into the same wells of a given well-plate.
The capillary position can be calibrated in the diagnosis mode of the ChemStation software especially for 384 well-plates.