This document describes the method of removing salts from protein samples prior to analysis on the Agilent 2100 bioanalyzer. This can improve the sensitivity and consistency of the analysis.
This document explains that high salt concentrations in protein samples can negatively affect the sensitivity of the Agilent 2100 bioanalyzer during electrokinetic injection. It details how using Pierce Protein Desalting Spin Columns to remove excess salts prior to analysis significantly improves system sensitivity and consistency. The note demonstrates that while high salt levels reduce signal intensity, they do not affect the accuracy of quantitation when using the Protein 200 Plus assay's internal standards.
| Desalting time | approximately 5 minutes |
|---|---|
| Salt removal efficiency | 95 % |
| Protein recovery | greater than 90 % |
| Sample volume range | 30 to 120 pl |
| Resin volume | approximately 700 pl |
High salt concentrations can affect the sensitivity of the system because salt is preferentially injected over proteins due to greater ionic strength.
No, desalting is primarily needed to improve sensitivity; quantitation remains accurate even with high salt due to the use of an internal standard.
The Pierce Protein Desalting Spin Columns are recommended for the quick removal of excess salts from samples.