Agilent 2100 Bioanalyzer Flow Cytometric Analysis Application Note

About this manual

The document details how to perform flow cytometric analysis using a small number of cells with the Agilent 2100 bioanalyzer. It explains that while traditional methods require high cell volumes, this microfluidic system allows for analysis with as few as 2,500 cells per sample by utilizing on-chip staining protocols.

What's inside

• Introduction
• Experiments and results
• Discussion and conclusion

Frequently asked questions

What is the minimum number of cells required to obtain appropriate quantitation for this experiment?

The document states that loading 2,500 cells onto the chip is sufficient for appropriate quantitation.

How long should the chips be incubated in the dark at room temperature?

After vortexing, the chips must be stacked and incubated in the dark for 20 minutes at room temperature.

What is the recommended number of cells for detecting subpopulations as low as 10% of the total population?

The recommended number of cells for this purpose is 20,000 cells per sample for most Agilent 2100 bioanalyzer cell applications.