This document introduces the importance of Agilent 2100 bioanalyzer in mRNA sample quality assessment, and provides detailed usage methods and precautions.
This document serves as a guide for interpreting data from the Agilent 2100 bioanalyzer when analyzing mRNA samples. It details how to assess sample quality, identify ribosomal RNA contamination, and recognize specific tissue profiles that may appear on electropherograms.
The text describes high-quality mRNA profiles as broad peaks, typically ranging between 500 and 9000 bases with a majority in the 1000-4000 base range. It also covers the identification of ribosomal RNA contamination, which appears as distinct peaks if it comprises five percent or more of the total concentration. Additionally, the document notes that certain tissues, such as chicken brain, may produce electropherograms with a second broad peak representing very long RNA transcripts.
The majority of transcripts in a high quality bovine lung sample are between 1000 and 4000 bases long.
The software identifies and quantitates ribosomal peaks that are five percent of the total mRNA concentration or greater.
A quick RNase digestion will reveal whether or not the peaks are RNA.