Agilent 1100 Series Off-line Multidimensional LC/MS Application Note

About this manual

The document presents an off-line two-dimensional liquid chromatography-mass spectrometry (2D LC/MS) approach as a technique to increase chromatographic resolution and protein identification results compared to fully automated on-line methods. The first dimension utilizes the Agilent 1100 Series micro fraction collection system for off-line fraction collection with strong cation exchange (SCX) chromatography.

The second dimension employs the Agilent Nanoflow Proteomics Solution combined with the Agilent Spectrum Mill MS Proteomics Workbench for LC/MS/MS analysis. The text details the specific equipment, software versions, and experimental conditions used for both off-line and on-line approaches when analyzing a Saccharomyces cerevisiae proteome.

What's inside

• Abstract
• Introduction
• Equipment
• Software
• Chromatographic method
• Sample preparation
• Results and discussion

Frequently asked questions

What is the stated purpose of the off-line multidimensional LC/MS approach described in this note?

The approach is presented as an effective tool for proteomics research that increases chromatographic resolution to improve protein identification results.

What specific Agilent system was used for fraction collection in the first dimension of the off-line method?

The Agilent 1100 Series micro fraction collection system was used for fraction collection in the first dimension.

What is the flow rate for the nanoflow pump used in the second dimension of the off-line approach?

The flow rate for the nanoflow pump in the second dimension is 300 nL/min.