This Application Note details how the separation of enantiomers, stereoisomers or diastereomers can be improved by recycle chromatography. The configuration of the system using two identical columns and a 2-position/6-port valve is shown, and the scope and limitations of the system are explained. An application example, which demonstrates the improvement in purity of the collected fractions when using recycle chromatography, is described.
The document describes a technique for improving compound purity by recycling compounds over two identical columns connected via a 2-position/6-port valve. This method reduces off-column dispersion compared to standard recycling techniques that pass compounds through the entire pump system.
An application example demonstrates the separation of steroid isomers using an Agilent Prep C18 column. The text provides specific experimental parameters, including mobile phases (water and acetonitrile), flow rates, injection volumes, and detector settings used in the experiment.
It improves the separation of enantiomers, stereoisomers, or diastereomers by recycling compounds over the same column until sufficient resolution is achieved.
The setup uses two identical columns and a 2-position/6-port valve to move the columns in the direction of the mobile phase flow.