Laboratory projects that require accurate analysis of PCR products can be divided into two classes: low throughput and high throughput.
This document describes the application of the Agilent 5100 Automated Lab-on-a-Chip Platform (ALP) to increase throughput in molecular biology projects, specifically for analyzing PCR products. The authors compare the ALP's performance against traditional agarose gels and the Agilent 2100 bioanalyzer, highlighting the ALP's ability to handle hundreds or thousands of samples efficiently. The text details four specific projects: mutation detection, viral pathogen screening, mycoplasma screening, and gene expression microarray development.
| Sample throughput per job | Up to 10 standard well plates (96 or 384 formats) |
|---|---|
| Simultaneous analysis | Four samples |
| Chip type used | Four-capillary DNA 1000 HT-4 chip |
| Run time per 96-well plate | Approximately 1.5 hours |
| Run time per sample (10 plates) | Below 1 minute |
The system provides simultaneous electrophoretic analysis of four samples.
Up to 10 sample plates can be handled during one job.
A four-capillary DNA 1000 HT-4 chip is used for the electrophoretic separation.