This document introduces the Agilent ZORBAX Rapid Resolution High Throughput HPLC columns using 1.8 µm particles that provide outstanding efficiency in short column lengths for high sample throughput of liquid chromatography and liquid chromatography/mass spectrometry methods. These columns can be used for either isocratic or gradient analyses with high quality, reliable results.
This document details the application of ZORBAX Rapid Resolution High Throughput (HT) HPLC columns, which utilize 1.8 µm particles, for high sample throughput in liquid chromatography and liquid chromatography/mass spectrometry (LC/MS). The text demonstrates how these columns maintain high resolution while significantly reducing analysis times compared to traditional columns.
Specific methods are provided for the analysis of clindamycin and lincomycin, an antibiotic and its precursor. The document outlines experimental conditions for both isocratic and gradient separations using narrow-bore and standard dimensions, highlighting the efficiency gains achieved with the Rapid Resolution HT technology.
| Column Length Range | 15-50 mm |
|---|---|
| Column ID (LC/MS) | 2.1 mm |
| Column ID (HPLC) | 4.6 mm |
| Flow Rate (LC/MS) | 0.5 mL/min |
| Flow Rate (HPLC) | 1.5 mL/min |
Yes, these columns can be used for either isocratic or gradient analyses with high quality results.
Short columns ranging from 15 to 50 mm are ideal for high sample throughput.