This study describes a method based on Agilent Multiple Affinity Removal System for removing high-abundance proteins from monkey plasma, which can increase the sensitivity of detecting low-abundance proteins.
This document describes an application of the Agilent Multiple Affinity Removal System for the immunodepletion of high-abundant proteins from cynomolgus monkey plasma. The study aims to increase the detection sensitivity of low-abundance proteins in proteomic analysis by removing abundant species like albumin and immunoglobulins.
| Target Proteins Removed | human albumin, transferrin, IgG, IgA, haptoglobin, and antitrypsin |
|---|---|
| Column Dimensions | 4.6 mm x 100-mm |
| Injection Volume | 200-uL |
| Flow Rate (Loading) | 0.5 mL/min |
| Flow Rate (Elution) | 1 mL/min |
| Molecular Weight Cutoff | 5 kDa |
| Sample Concentration | 250 ug |
The system removes human albumin, transferrin, IgG, IgA, haptoglobin, and antitrypsin.
Yes, the study demonstrates that the system for human proteins can be applied for effective removal of high-abundant proteins from monkey plasma.
Removal enhances the detection and resolution of less-abundant proteins and aids in the display of proteins that are otherwise obscured.