Agilent G1540N/G3243A Confirmation of THC in Oral Fluids Using High-Resolution 2-D GC/MS Application Note

About this manual

Oral fluids are increasingly used as an alternative matrix to urine for drug testing. This document details a method to detect drugs of abuse, specifically THC, at concentrations down to 0.5 ng/mL in diluted oral fluid using high-resolution two-dimensional gas chromatography/mass spectrometry (2-D GC/MS). The technique overcomes interference problems from complex sample matrices that often complicate standard analysis.

The described system utilizes a standard quadrupole GC/MSD configuration enhanced with a Deans switch and an air-cooled focusing trap. Samples are separated on a nonpolar DB-1ms column, then heart-cut to a polar DB-17ms column for further separation. This approach provides the required selectivity and sensitivity comparable to MS/MS techniques while remaining simpler and less expensive.

What's inside

• Abstract
• Introduction
• GC/MSD Configuration
• Oral Fluid Sample Collection and Preparation
• Deans Switch Operation

Frequently asked questions

What concentration of THC must be detected in oral fluid samples collected with the Intercept oral fluid collector?

The confirmation technique must detect THC down to 0.5 ng/mL of diluted oral fluid.