This article describes the application of scanning probe studies on a metalloprotein, with a focus on the analysis and control of biological oxidases, as well as the generation of biosensing systems targeting specific compounds.
This document details research conducted by the University of Oxford using Agilent AFM instrumentation to study metalloproteins. The study focuses on analyzing and controlling biological redox proteins and enzymes, such as azurin and cytochrome P450 monooxygenases, with electrode surfaces.
The authors developed a strategy to anchor proteins and enzymes to gold electrodes using site-specifically introduced surface cysteine residues. This methodology enabled high-resolution in situ scanning tunneling microscopy (STM) studies and electrochemical probing of the protein/enzyme adlayers under quasi-physiological conditions.
The ambient scanning tunneling images in Figure 3 were taken with a bias voltage of 1.1 V and a tunnel current of 190 pA.
The fluid phase tunneling image in Figure 4 used a scan size of 120 x 120 nm, a bias voltage of 1.5 V, and a tunnel current of 200 pA.