This paper compares the Agilent J&W DB-5ms Ultra Inert column with similar versions from four other column manufacturers with respect to their chromatographic behaviors for active analytes. Superior results were observed on the DB-5ms columns for basic compounds. Chromatograms depicting peak shape characteristics are presented to show how exceptional inertness leads to a significant reduction in tailing of active basic compounds for more accurate, reliable analytical results. Excellent chromato- graphic performance was obtained for a diverse group of compounds that included chlorophenols, organophosphorus pesticides and aromatic positional isomers demon- strating the applicability of the DB-5ms column for a wide range of applications.
This document presents a comparative study on the chromatographic behavior of Agilent J&W DB-5ms Ultra Inert columns versus similar columns from four other manufacturers. The analysis focuses on the inertness required for analyzing very active samples, specifically basic compounds that are prone to tailing due to acidic sites in non-inert columns.
The study utilized an Agilent 6890N GC system to test a mixture of six basic compounds, chlorophenols, organophosphorus pesticides, and aromatic positional isomers. Results demonstrated that the DB-5ms Ultra Inert column provided superior peak shapes with significantly reduced tailing factors compared to the competitor columns, ensuring more accurate and reliable analytical results.
The study analyzed a mixture containing Triethylamine, Pyridine, 4-Methylpyridine, N.N-Dimethylacetamide, 2,4-Dimethylaniline, and Dicyclohexylamine.
The column dimensions are 30 m x 0.25 mm x 0.25 um.
The oven program heats to a final temperature of 240 °C.