The study proposes an optimized method for the analysis of tropane alkaloids by CE-ESI-MS. The results show that the method has high selectivity and sensitivity and is suitable for the quantitative analysis of tropane alkaloids in biological samples.
This document describes a method for the quantitative evaluation of isomeric composition in pharmacokinetic samples using Capillary Electrophoresis (CE) coupled with Electrospray-Ionization Mass Spectrometry (ESI-MS). The study focuses on the analysis of tropane alkaloids, specifically atropine and scopolamine, found in Solanaceae plants like Datura seeds. The method utilizes chiral selectors, such as cyclodextrins, to separate racemic mixtures, with detection limits achieved in the mid-ppb range.
| Injection time | 10 sec |
|---|---|
| Capillary length | 90 cm |
| Capillary inner diameter | 50 um |
| Buffer composition | 50 M formic acid, 200 mM trimethyl-CD |
| Applied voltage | 30 kV |
| Temperature | 20°C |
| Sheath liquid flow rate | 4 pL/min |
| Neubulizing gas pressure | 10 psi |
| Drying gas flow rate | 6 L/min |
| Drying gas temperature | 300 °C |
| SIM m/z values | 209.2, 304.1 |
Detection limits in the mid-ppb range could be achieved using the optimized method parameters.
The reproducibility was less than 1% RSD for migration time.
The assay was linear in a range of 1-100 mg/L.