This document describes the analysis of 19 pharmaceuticals in positive ion mode and 11 pharmaceuticals in negative ion mode using solid phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The analysis was performed at low picogram level on column without any derivatization. Good linearity was observed for analytes from 1 pg to 1 ng on column. Repeatability from six injections of analytes at 5 pg on column showed RSDs below 15%, for all target compounds except for fluoxetine at 23%.
The document details an analytical method developed by the U.S. Geological Survey National Water Quality Laboratory, implemented with Agilent Technologies instrumentation to detect trace pharmaceuticals in surface and ground water. The study focuses on monitoring man-made chemicals that disrupt endocrine systems.
Specific procedures for sample preparation using Oasis HLB solid-phase extraction (SPE) are provided. The analysis utilizes a ZORBAX Extend-C-18 column coupled with an Agilent 1200 LC system and G6410A Mass Spectrometer, operating in both positive and negative ion modes via Electrospray Ionization (ESI).
The method analyzes 19 pharmaceuticals using the positive ion mode.
Analytes were successfully analyzed at a low picogram level of 1 pg on column.
The repeatability for fluoxetine showed an RSD of 23%.