This paper describes a method for analysis of organochlorine pesticides (OCPs) in environmental remediation samples using 0.18 mm id high-efficiency GC columns (20 m×0.18 mm id×0.18µm film thickness) and helium carrier gas. DB-17ms stationary phase is used for primary analysis and DB-XLB stationary phase for confirmation. Using this method, primary analysis and confirmation of 22 CLP pesticides in the protocol is achieved in an 11-minute analysis, a 35% reduction in analysis time versus 0.32 mm id columns.
This document describes a method for analyzing 22 Contract Laboratory Program (CLP) pesticides using high-efficiency Gas Chromatography (GC) columns. The method utilizes 0.18 mm ID columns with DB-17ms and DB-XLB stationary phases to achieve primary analysis and confirmation in an 11-minute run time, representing a significant reduction compared to standard column widths.
The note details the experimental setup using an Agilent 6890N GC equipped with dual nECDs. It highlights the use of helium as a carrier gas for safety and compatibility reasons, providing specific chromatographic conditions including oven temperature programs and flow rates. Additionally, it explains how to use Agilent's method translation software to convert existing methods originally developed for hydrogen carrier gas into optimized protocols suitable for helium operation.
Helium carrier gas is selected for the demonstration to show utility with high-efficiency columns and ensure full compatibility with standard gas chromatographs.
The primary analysis and confirmation of 22 CLP pesticides are achieved in an 11-minute analysis.
Agilent's method translation software is used to convert original conditions with hydrogen carrier gas to new conditions using helium.