This article introduces an ESI-LC/MS/MS analysis method of dansyl derivatized phytoestrogens on an Agilent ZORBAX RRHT Eclipse Plus C18 1.8µm column. This method has high sensitivity and accuracy, and can be used to analyze a variety of phytoestrogens.
Phytoestrogens are naturally occurring plant compounds with potential health benefits. This document details a method for analyzing these compounds by derivatizing them with dansyl chloride to enhance sensitivity in LC/MS/MS analysis compared to previous APCI methods.
The study utilizes an Agilent 1200 Series Rapid Resolution LC system coupled with an Agilent 6410 Triple Quadrupole Mass Spectrometer. The method employs a specific ammonium acetate/acetonitrile gradient on an Agilent ZORBAX RRHT Eclipse Plus C18 1.8 µm column to achieve rapid separation and high sensitivity, achieving signal-to-noise ratios greater than 10 for the lowest calibrator.
The flow rate was set to 0.5 mL/min.
The thermostatted column compartment was set to 40 °C.
The vials were heated at 60 °C for 10 minutes.