Agilent PL-GPC 50 Plus Application Note: Analysis of Cornflour by GPC with Viscometry

This article introduces the characteristics of cornflour and its applications in the food and plastics industries. The molecular structure differences of cornflour from different sources and the impact of linear amylose and branched amylopectin on its viscosity were investigated using GPC analysis.


Agilent PL-GPC 50 Plus Application Note: Analysis of Cornflour by GPC with Viscometry - cover page
Brand
Agilent
Category
Other
Document type
Application Note
Language
English
Pages
3
File format
PDF
File size
537 KB
Published
12 June, 2012
Updated
28 September, 2023
MD5 checksum
59791B79E0FB0F51219868B5E09D81BA

About this manual

This document details the analysis of cornflour samples to investigate variations in amylose and amylopectin content. The study utilized an integrated GPC system to determine molecular weight distributions using the Universal Calibration method and compared sample structures using Mark-Houwink plots.

What's inside

  • Introduction
  • Instrumentation
  • Materials and Reagents
  • Results and Discussion
  • Conclusion

Specifications

Flow Rate1 mL/min
Temperature50 °C
EluentDimethyl sulfoxide + 0.1% lithium bromide
Columns3x PLgel 10 ym MIXED-B 300 x 7.5 mm

Frequently asked questions

What instrument was used to analyze the cornflour samples?

The samples were analyzed by an Agilent PL-GPC 50 Plus fitted with an Agilent PL-BV 400RT viscometer.

How was the structure of the cornflour samples compared?

The structure was compared using the Mark-Houwink plot of log (intrinsic viscosity) as a function of log (molecular weight).

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