Synthesis of HIV-1 gp41 using AmphiSpheresTM Resin
This document details the synthesis of the 28-residue HIV-1 gp41 ectodomain sequence using Agilent AmphiSpheres 40 RAM resin. The peptide was prepared on a 0.1 mmol scale using a peptide synthesizer with Fmoc-AA-OH and HBTU coupling reagents. Following assembly and cleavage from the resin using TFA/TIPS/water, the peptide was purified and analyzed via HPLC and MS.
| Column | Varian Polaris 5 ym C18-A 150 x 4.6 mm |
|---|---|
| Gradient | 10 — 30% MeCN (0.1% TFA), 0 - 30 min |
| Flow rate | 1 mL/min |
| UV detector | 220 nm |
| Injection volume | 20 uL |
| Scale | 0.1 mmol |
The synthesis was performed using AmphiSpheres 40 RAM resin.
AmphiSpheres resin outperformed commercially available polyether resin, as confirmed by HPLC/MS.
The major peak was confirmed as 3653 [M+H]+.