Agilent S!-01998 Synthesis of ampR1 Primer using StratoSpheres DNA Application Note

About this manual

The document details the synthesis of the ampR1 (or SR1) reverse primer, a 20-mer sequence recommended for sequencing and amplification with the pSMART cloning vector. It compares the performance of StratoSpheres DNA dG support against commercial PS/DVB and controlled pore glass (CPG) supports.

What's inside

• Introduction
• Oligonucleotide Synthesis
• HPLC Analysis

Frequently asked questions

What is the recommended sequence for the ampR1 reverse primer?

The recommended sequence is 5' CTT TCT GCT ATG GAG GTC AGG TAT G 3'.

What temperature and duration are required for deprotecting the side chain protecting groups?

Deprotection is accomplished by heating to 55 °C overnight.

What is the flow rate used during the HPLC analysis?

The flow rate is set to 1.5 mL/min.