This application note describes a method for synthesizing Leu-enkephalin using AmphiSpheresTM resin.
This document details the synthesis of Leu-enkephalin (H-Tyr-Gly-Gly-Phe-Leu-NH2) using AmphiSpheres 20 RAM resin on a 0.1 mmol scale. The peptide was prepared using a peptide synthesizer with Fmoc-AA-OH and HBTU coupling reagent. Following assembly, the peptide was cleaved from the resin using TFA / TIPS / water and purified via rotary evaporation and trituration with cold ether.
| Synthesis Scale | 0.1 mmol |
|---|---|
| Coupling Reagent | HBTU |
| Deprotection Reagent | 20% piperidine in DMF |
| Cleavage Reagents | TFA / TIPS / water 95:2.5:2.5 |
| Cleavage Time | 2-3 h |
| Resin Type | AmphiSpheres 20 RAM |
| Resin Composition | hydrophobic polystyrene and hydrophilic polyethyleneglycol |
No, NMR analysis showed absolutely no sign of PEG chains arising from the resin.
It contains hydrophobic polystyrene and hydrophilic polyethyleneglycol components.