This white paper describes the synthesis of d(T)50 using Varian StratoSpheres DNA support.
The document details an experiment synthesizing poly-T oligomers to evaluate the pore structure and accessibility of solid-phase supports. It highlights that while controlled pore glass (CPG) yields are compromised by incomplete coupling, the StratoSpheres DNA support exceeds CPG performance and matches leading commercial macroporous PS/DVB standards.
The experimental procedure involved preparing oligonucleotides on a 0.2 UM scale using an Applied Biosystems 392 DNA/RNA Synthesizer with standard chemistry. Following synthesis, the oligomers were cleaved with ammonium hydroxide and analyzed via HPLC using a Pellicular SAX column at 60 °C.
Synthesizing poly-T oligomers reveals information about the pore structure and pore accessibility of the support during synthesis.
The oligonucleotides were prepared on a 0.2 UM scale.
The flow rate used during HPLC analysis was 1.5 mL/min.