This article introduces a simple method for the determination of copper, zinc, iron, calcium, magnesium, sodium, and potassium in serum using flame atomic absorption spectroscopy. One milliliter of serum is diluted to 10 milliliters with deionized water, and the determination of seven elements is automatically performed.
This document presents a simplified procedure for the determination of Copper (Cu), Zinc (Zn), Iron (Fe), Calcium (Ca), Magnesium (Mg), Sodium (Na), and Potassium (K) in serum. The method utilizes a SpectrAA-40 atomic absorption spectrometer with a programmable sample changer to automate the process, reducing the need for complex dilutions and anti-interference reagents compared to traditional methods.
The note details the specific instrument parameters, such as wavelength, slit width, and burner height, for each element. It also outlines the preparation of standard solutions and the procedure for analyzing serum samples diluted with deionized water. Results regarding recovery rates and precision are provided, along with a discussion on the effects of different dilution ratios and the comparison of this method against others.
| Instrument Model | SpectrAA-40 |
|---|---|
| Sample Changer | PSC-56 |
| Printer | Epson LX-80 |
| Cu Wavelength | 324.8 nm |
| Zn Wavelength | 213.9 nm |
| Fe Wavelength | 248.3 nm |
| Ca Wavelength | 422.7 nm |
| Mg Wavelength | 202.5 nm |
| Na Wavelength | 330.3 nm |
| K Wavelength | 404.4 nm |
| Acetylene Flow | 13.5 L/min |
| Air Flow | 13.5 L/min |
| Measurement Time | 150 measurements in one hour |
The method determines Copper (Cu), Zinc (Zn), Iron (Fe), Calcium (Ca), Magnesium (Mg), Sodium (Na), and Potassium (K).
One milliliter of serum is diluted to 10 milliliters with deionized water.
The procedure can be completed automatically with 150 measurements in one hour.
The analysis uses a SpectrAA-40 atomic absorption spectrometer with a PSC-56 programmable sample changer.