This document is about the use of the Agilent 7696A Sample Prep WorkBench, which describes how to use the tool to automate the serial dilution of samples and measure the performance of flame ionization detectors.
This document details a method to automate the preparation of a sample set by serial dilution using the Agilent 7696A Sample Prep WorkBench. The process involves preparing a series of samples, each diluted by a factor of ten from the previous one, to measure Flame Ionization Detector (FID) performance metrics such as linearity, sensitivity, and minimum detectable level (MDL). The note compares automated preparations against manual methods.
| Syringe capacity (Back injector) | 500-uL |
|---|---|
| Syringe capacity (Front injector) | 100-pL |
| Dilution factor | Tenfold |
| Mixing speed | 1500 RPM |
| Mixing duration | 0 min 5 sec |
| Runtime (1 mL set) | 49 min |
| Runtime (0.5 mL set) | 41 min |
To measure Flame Ionization Detector (FID) performance, specifically linearity, sensitivity, and minimum detectable level (MDL).
A set of seven or eight samples is required to demonstrate the normal seven orders of magnitude of FID linearity.
The first protocol uses 900 uL solvent + 100 uL sample, while the second uses half these amounts: 450 uL solvent + 50 uL sample.