This Application Note describes a method for simultaneous determination of eight FDA/EP approved active UV filter ingredients in under two minutes using the Agilent 1290 Infinity LC and Agilent Poroshell columns. The method offers short analysis time, high sensitivity, robustness and good resolution, and is suitable for qualitative and quantitative analysis of active UV filters in commercial personal care products.
The document presents an analytical method developed by Agilent Technologies India Pvt. Ltd. for the simultaneous determination of eight FDA/EP approved organic UV filter ingredients found in personal care products. The primary goal is to provide a short, sensitive, and robust separation technique that significantly reduces analysis time compared to traditional methods.
| Analysis Time | 99 seconds |
|---|---|
| Column Dimensions | 75 mm x 2.1 mm, 2.7 um |
| Column Temperature | 55 C |
| Flow Rate | 1 mL/min |
The method achieves separation of eight organic UV filters in 99 seconds.
An Agilent Poroshell 120 EC-C18 column with dimensions 75 mm x 2.1 mm and particle size 2.7 um.
The detector was programmed for three different wavelengths: 288 nm, 304 nm, and 358 nm.