This application note presents a method for the analysis of adulterants in weight loss supplements marketed as “all natural.” The analysis is performed using the Agilent 500 Ion Trap LC/MS with Turbo Data Dependent Scanning (TurboDDS), which performs full scan and MS/MS analysis in a single injection. Target adulter- ants were observed and yielded spectra with excellent library matches to the NIST MS Library when spiked into a dietary supplement matrix at 10 µg/mL and then diluted 1:10 in water. A real incurred weight loss product was also analyzed and found to be adulterated with Bumetanide. Unlike other techniques that use targeted analysis to detect known adulterants in a dietary supplement of interest, TurboDDS screens and identifi es both known and unanticipated adulterants in a single experiment.
This document presents a method for analyzing adulterants in dietary supplements marketed as "all natural" using the Agilent 500 Ion Trap LC/MS with Turbo Data Dependent Scanning (TurboDDS). The analysis targets common adulterants such as loop diuretics, appetite suppressants, and respiratory drugs.
| Column | 2.0 mm x 150 mm Pursuit XRs 3 yp C18 |
|---|---|
| Flow rate | 0.200 mL/min |
| Ionization mode | ESI positive and negative |
| API drying gas | 30 psi at 350 °C |
| Scan range | 50-500 m/z |
The analysis targeted loop diuretics (Bumetanide and Furosemide), appetite suppressants (Sibutramine, Rimonabant, and NIDA), a respiratory drug (Theophylline), a laxative (Phenolphthalein), and an antiepileptic drug (Phenytoin).
Unlike targeted analysis which only detects known compounds, TurboDDS performs full scan surveying and MS/MS data collection simultaneously to identify both known and unanticipated adulterants.
The standard samples were made at 10 pg/mL and then diluted 1:10 in water for a final concentration of 1 g/mL.