This document highlights the method and advantages of using the Agilent Cary 60 UV-Vis spectrophotometer for determining drug tablet concentration in pharmaceutical applications.
This document details the use of the Agilent Cary 60 UV-Vis spectrophotometer equipped with fiber optic probes for determining the concentration of active pharmaceutical ingredients (API) in drug tablets. It highlights the benefits of in situ measurements for drug development and production, offering time and cost savings compared to conventional sample transfer systems.
The note presents a case study involving the analysis of an anti-malarial API. Using the WinUV software's Concentration module, the document demonstrates the instrument's ability to generate linear concentration curves with high reproducibility. It concludes that this approach is critical for determining precise drug concentrations and supports regulatory compliance through 21 CFR part 11 capable software.
| Instrument Model | Cary 60 UV-VIS Spectrophotometer |
|---|---|
| Probe Type | Torlon fiber optic probe |
| Probe Coupler | Torlon fiber optic probe coupler |
| Correlation Coefficient | 0.99976 |
| Absorbance Range | 0-2 Absorbance units |
The WinUV software contains the 21 CFR part 11 module that assists with meeting regulatory requirements.
The calibration equation is Abs = 0.00688*Conc -0.01056.
Methods were adapted in-house from those described in British Pharmacopoeia 2011.