The performance of the Agilent 500 Ion Trap LC/MS in EPA Method 535 is evaluated in terms of calibration and quantitative accuracy in a variety of matrices. The data reveals this instrument provides reliable quantitative data, even with the presence of highly concentrated surface water extracts.
The document evaluates the performance of the Agilent 500 Ion Trap LC/MS in EPA Method 535, specifically focusing on the determination of herbicide degradates (ethanesulfonic acids and oxanilic acid) in drinking water. The study assesses calibration and quantitative accuracy using surrogate standards in various matrices, including reagent water, finished tap water, and surface water extracts.
The analysis utilizes LC/MS/MS with electrospray ionization (ESI) negative mode. The note details the specific instrumentation setup, including the Agilent ProStar 210 Binary Solvent Delivery Modules and ProStar 420 AutoSampler. It provides specific HPLC gradient conditions and MS parameters, demonstrating that the instrument provides reliable quantitative data even with highly concentrated surface water extracts containing dissolved organic carbon.
| Transition (Surrogate) | 300 > 121 m/z |
|---|---|
| API drying gas temp | 400 °C |
| API drying gas pressure | 35 psi |
| Scan range | 119-123 m/z |
The study evaluates the Agilent 500 Ion Trap LC/MS equipped with an electrospray ionization source (ESI) and built-in syringe pump.
The surrogate standard used was Dimethachlor ESA.
The standard calibration curve for SS-Dimethachlor-ESA showed an excellent correlation factor (r²) value of 0.9999.
Yes, the recovery criteria were between 70-130%, which is in agreement with the EPA 535 method.