The application note demonstrates how the Cary 60 is used to measure the purity of DNA at 4 °C using the micro fiber optics accessory – resulting in significant time and cost savings without compromise in the accuracy and reproducibility of data quality.
The document describes an application for the Agilent Cary 60 UV-Vis spectrophotometer that allows users to measure small volumes of biological samples, such as DNA, directly from their storage environment at 4 °C. By using the fiber optics microprobe accessory, the instrument can be brought to the sample rather than requiring the sample to be warmed up or transferred to a cuvette.
The system can analyze samples as low as 40 uL.
The instrument can read samples between 4 and 110 °C.
The correlation coefficient is 0.9976.