Agilent Isocratic Purification of Synthetic Acyl Carrier Protein Fragment 65-74 Application Note

This application note describes the use of Agilent PLRP-S 100Å reversed phase media for the purification of synthetic acyl-carrier protein (ACP) fragment 65-74.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 669 KB
Published: 11 June, 2012
Updated: 30 September, 2023
Model: PLRP-S 100A
MD5 checksum: D41AFC805E2C9D99A48F928C50C92464
PDF: Agilent Isocratic Purification of Synthetic Acyl Carrier Protein Fragment 65-74 Application Note PDF

About this manual

This document details the use of Agilent PLRP-S media for the purification of synthetic biomolecules, specifically focusing on a synthetic Acyl Carrier Protein (ACP) fragment 65-74. The text explains that the chemical and thermal stability of PLRP-S makes it suitable for batch purification requiring aggressive column clean-up.

What's inside

Introduction
Conditions
Results and Discussion
Conclusion

Specifications

Part Number	PL1512-3800
Eluent	0.1% TFA in 20% ACN / 80% water
Flow Rate	1.0 mL/min
Detection	UV, 220 nm

Frequently asked questions

What media is used for the purification?

Macroporous, rigid polystyrene/DVB-based reversed phase media, PLRP-S.

What is the maximum amount of crude synthetic ACP 65-74 chromatographed?

Approximately 1 mg of crude synthetic ACP 65-74 can be chromatographed isocratically.

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