This report describes a method by which a crude sample of solid phase synthesized leuprolide can be purified on a PLRP-S 100Å 10 μm column.
This document details a method for purifying leuprolide, a gonadotropin-releasing hormone agonist, using preparative scale chromatography. The study focuses on the use of a PLRP-S 100A 10 um column to achieve high purity through concentration overload techniques.
| Column Type | PLRP-S 100A 10 um |
|---|---|
| Column Dimensions (Analytical) | 250 x 4.6 mm |
| Column Dimensions (Preparative) | 250 mm x 1 in. |
| Flow Rate | 1 mL/min |
| Detection Wavelength | 220 nm |
| Achieved Purity | 98.3% |
| Achieved Yield | 46.8% or 82.4% |
A PLRP-S 100A 10 um column was used for the purification.
The sequence is p-Glu-His-Trp-Ser-Tyr-D-Leu-Leu-Arg-Pro-NHEt.
The conditions were 0.1% TFA in 28% ACN and 72% water.