This document describes the extraction of polar basic drugs from plasma using polymeric solid phase extraction cation exchange technique. The technique allows for rapid and efficient removal of neutral and acidic interferences, as well as concentration of basic analytes, resulting in improved analytical performance and sensitivity in the quantification of basic compounds.
The document details the use of Agilent Bond Elut Plexa PCX, a polymeric cation exchange sorbent, for the extraction of polar basic pharmaceutical drugs from human plasma. This technique is designed to remove neutral and acidic interferences while concentrating basic analytes, thereby improving analytical performance and sensitivity compared to reversed-phase sorbents.
| Sorbent Type | Polymeric cation exchange |
|---|---|
| Plate Format | 96 well plate |
| Part Number | A496801 0 |
| Limit of Detection (LOD) | 1.0 ng/mL |
| Column Used | Agilent Pursuit 018 3 ym, 2.0 x 50 mm |
It is ideal for extracting polar basic drugs and compounds with basic amino functional groups from plasma.
It removes neutral and acidic interferences, concentrates basic analytes, and significantly reduces ion suppression.
The sample mixture should be loaded at a flow rate of 1 mL/min.