This application note provides detailed information on how to use Agilent PL-GPC 50 Plus to perform GPC with viscometry on starches. The column, mobile phase, detectors, and other configurations are introduced. The separation results of two starch samples are also analyzed.
Starches are polysaccharides used in various industrial applications, particularly in food. The molecular weight distribution determines their final properties and suitability for specific uses like confectionary or processed meats.
This document details an investigation into two starch samples using Gel Permeation Chromatography (GPC) coupled with viscometry on the Agilent PL-GPC 50 Plus system. The analysis utilized mixed-bed columns, a differential refractive index detector, and a viscometer to determine molecular weight distributions and structural differences between corn and potato starches.
The information obtained by employing a viscometer can highlight structural and/or chemical differences between the polymers.
The flow rate used for the analysis was 1.0 mL/min.
The sample concentration used for the analysis was 2.0 mg/mL.