Fast (5 min), dilute-and-shoot method for the determination of morphine and one of its major metabolites (morphine-glucuronide) in biological fluids (saliva and urine) using the Agilent 500 Ion Trap LC/MS
This document presents a rapid (5-minute) method for determining morphine and morphine-glucuronide in biological fluids using the Agilent 500 Ion Trap LC/MS. The method utilizes a dilute-and-shoot approach that eliminates the need for lengthy derivatization processes typically required in GC/MS methods, making it suitable for high-throughput screening environments.
The application details the specific instrumentation, including the Agilent 500 Ion Trap LC/MS with ESI source, ProStar 210 Solvent Delivery System, and CTC Analytics HTS PAL AutoSampler. It provides comprehensive sample preparation instructions, mass spectrometry and LC conditions, and discusses the use of a diverter valve to protect the instrument from high salt concentrations in urine samples.
| Analysis Time | 5 min |
|---|---|
| Morphine Limit of Detection | 10 pg |
| Morphine Limit of Quantification | 20 pg |
| Morphine-Glucuronide Limit of Detection | 40 pg |
| Morphine-Glucuronide Limit of Quantification | 60 pg |
| Column | Agilent Pursuit C18 3 mm 100 x 2mm |
| Flow Rate | 0.2 mL/min |
No, the method does not require lengthy derivatization processes.
The method is designed for saliva and urine.
The analysis is rapid, taking approximately 5 minutes.