This application note describes the conditions and results for the separation of Epikote 828 and 1001 using an Agilent HPLC system.
This document details the High-Performance Liquid Chromatography (HPLC) analysis of Epikote 828 and Epikote 1001 epoxy resins. It explains that PLRP-S, a rigid macroporous styrene/divinylbenzene phase, is ideal for assessing the oligomeric composition of these resins due to its chemical stability and robustness across various eluents.
The note provides specific analytical conditions, including a sample concentration of 1.3 mg/mL, a PLRP-S column (p/n PL1512-5801), and a specific gradient elution profile using water, acetonitrile, and tetrahydrofuran. Results indicate that Epikote 828 contains greater quantities of smaller oligomers compared to Epikote 1001, which contains larger quantities of higher oligomers.
| Sample Concentration | 1.3 mg/mL |
|---|---|
| Column | PLRP-S 300A 8 ym, 250 x 4.6 mm |
| Column Part Number | PL1512-5801 |
| Eluent A | 100% water |
| Eluent B | 90% ACN, 10% THF |
| Detection Wavelength | 254 nm |
The PLRP-S column is recommended for the assessment of the oligomeric composition of epoxy resins.
Epikote 828 contains greater quantities of smaller oligomers, while Epikote 1001 contains larger quantities of higher oligomers.
Yes, it is possible to switch between organic modifiers such as methanol and tetrahydrofuran.