This application note describes the use of the Agilent 2100 bioanalyzer to quickly verify siRNA integrity and determine optimal transfection conditions for gene silencing experiments.
This document outlines a method for optimizing transfection conditions for small interfering RNA (siRNA) using the Agilent 2100 bioanalyzer. It details how to verify siRNA integrity and purity using the DNA 1000 LabChip kit and evaluate transfection efficiency and cell viability using the Cell Fluorescence LabChip kit with fluorescent probes.
The single-stranded sequences should be incubated in siRNA Buffer for one minute at 91 °C.
Cells must be incubated for 20 minutes in the dark with the life staining dye.
The streamlined protocol allows the entire effect analysis to be completed in 60 minutes.