This study explored the interference of Sybr®Green I on the performance of Agilent 2100 bioanalyzer. The results showed that Sybr®Green I had little effect on the performance of the analyzer.
This document presents a study testing whether the presence of Sybr Green I, a common intercalating dye used in real-time PCR, affects the performance of the Agilent 2100 bioanalyzer. The authors investigated if this dye interferes with the Lab-on-a-Chip technology's ability to measure fragment size and purity.
The results demonstrate only minor influences of Sybr Green I on the performance of the Agilent 2100 bioanalyzer.
Sybr Green I causes a slight decrease in the apparent amplicon size as detected by the DNA 1000 assay.
The presence of Sybr Green I leads to an increase in the computed concentration of PCR products.