This application note describes how the Agilent Technologies 2100 Bioanalyzer can be used to analyze protein extracts from transgenic seedlines. Accuracy and precision in the determination of protein size and concentration was sufficiently good to allow for the characterization of experimental seedlines based solely on expressed protein profiles.
The document details an application where the Agilent 2100 Bioanalyzer is used to characterize the expression of conglycinin (7S) and glycinin (11S) proteins in soybean seedlines. The study compares unknown transgenic lines against wild-type controls that preferentially express either the 7S or 11S protein groups.
Samples can be loaded, separated, and analyzed for relative protein composition in less than 45 minutes.
The Protein 200 LabChip has a size range of 14-200 kDa.
The average ratio for the 20 unknown extracts was 0.72 ±0.1.