This application note demonstrates the superior separation ability of Agilent Hi-Plex H in the analysis and quantification of the organic acids in wine, by comparison with a column from another manufacturer.
The document introduces the Agilent Hi-Plex H column, a high-performance ligand-exchange chromatography column based on polystyrene/divinylbenzene (PS/DVB). It is designed for analyzing sugars, sugar alcohols, and organic acids, offering improved efficiency and reproducibility compared to alternative columns.
The note details an investigation into the analysis of organic acids in wine samples. By comparing the Agilent Hi-Plex H column against a standard crosslinked PS/DVB column from another manufacturer, the document highlights the superior separation ability of the Hi-Plex H, particularly in resolving closely eluting compounds like malic acid and glucose.
| Column Dimensions | 7.7 x 300 mm |
|---|---|
| Particle Size | 8 µm |
| Crosslinking | 8% |
| Counter Ion | Hydrogen |
| Operating Temperature | 75°C |
| Flow Rate | 0.4 mL/min |
The main organic acids identified for analysis include acetic acid, lactic acid, succinic acid, malic acid, citric acid, and tartaric acid.
The document recommends using dilute mineral acid as the eluent for the analysis of organic acids.
The Hi-Plex H provides superior separation ability and resolution, specifically resolving malic acid and glucose which often co-elute on alternative crosslinked PS/DVB columns.