Agilent 6460 Triple Quadrupole LC/MS Pesticide Analysis Application Note

About this manual

The document details a method using triggered Multiple Reaction Monitoring (tMRM) to analyze 51 pesticides in food matrices. It highlights the use of an Agilent 6460 Triple Quadrupole LC/MS coupled with an Agilent 1290 Infinity LC system.

Two specific cases of false positive identifications are explored: tebuthiuron in chamomile and tebufenpyrad in ginger. The text explains how tMRM acquisition, combined with library matching, differentiates endogenous matrix compounds from actual pesticide contaminants by analyzing additional product ions and verifying retention time tolerances.

What's inside

• Introduction
• Sample Preparation
• LC/MS Analysis
• Results and Discussion
• tMRM Acquisition

Frequently asked questions

What is the maximum allowed retention time variance for analyte identification?

The retention time of the analytes must not vary beyond 2.5 %.

How many replicate injections were used to determine the precision of tebuthiuron at 10 ppb?

Five replicate injections of tebuthiuron at 10 ppb spiked into chamomile extract had a %RSD value of 0.94.

What is the maximum number of concurrent MRMs allowed in this analysis?

The system allows a maximum of 84 concurrent MRMs.