Agilent Hi-Plex H is a high-performance ligand-exchange chromatography column that can be used for quantitative analysis of organic acids in red wine, white wine, rosé wine, and dessert wine.
This document describes the application of the Agilent Hi-Plex H ligand-exchange chromatography column for the quantitative analysis of organic acids and sugars in wine. The Hi-Plex H column, based on polystyrene/divinylbenzene with an 8% crosslinking and hydrogen counter ion, offers improved efficiency and reproducibility for analyzing compounds like acetic, lactic, succinic, malic, citric, and tartaric acids.
The note details a specific method where wine samples (red, white, rosé, and dessert) were directly injected onto the column without pretreatment. Using a refractive index detector, the method successfully distinguished between different wine types based on their unique profiles of organic acids and sugars, such as high malic acid and fructose levels in dessert wines versus high lactic acid in red and white wines.
| Column Dimensions | 7.7 x 300 mm |
|---|---|
| Mobile Phase | 0.004 M H2SO4 |
| Flow Rate | 0.4 mL/min |
| Temperature | 75°C |
| Detector | RI |
It significantly reduces the need for complicated sample preparation involving elution through an ion-exchange resin bed.
Yes, each wine was directly injected onto the column without any sample pretreatment.
The method analyzes acetic acid, lactic acid, succinic acid, malic acid, citric acid, and tartaric acid.