Introduces the separation of four metanephrines in urine with an Agilent Polaris C18-A column
This document details a High-Performance Liquid Chromatography (HPLC) method for separating four specific metanephrines found in urine samples. The method utilizes an Agilent Polaris C18-A column and electrochemical detection (ECD) to analyze both standard and patient urine samples.
The procedure specifies the use of a mobile phase containing 0.1 M KH2PO4, pH 6.0, 20 mg/L 1-octanesulfonic acid, sodium salt, 1 g/L sodium EDTA, and 6% acetonitrile. The analysis is performed at a flow rate of 1.0 mL/min with an injection volume of 10 uL at a temperature of 30°C.
| Column | Agilent Polaris C18-A, 4.6 x 150 mm, 5 um |
|---|---|
| Mobile phase | 0.1 M KH2PO4, pH 6.0, 20 mg/L 1-octanesulfonic acid, sodium salt, 1 g/L sodium EDTA, 6% acetonitrile |
| Flow rate | 1.0 mL/min |
| Injection volume | 10 uL |
| Detector | ECD, Antec Decade, +0.63 V Glassy Carbon — Ag/AgCl |
An Agilent Polaris C18-A column is used for the HPLC separation.
The mobile phase consists of 0.1 M KH2PO4, pH 6.0, 20 mg/L 1-octanesulfonic acid, sodium salt, 1 g/L sodium EDTA, and 6% acetonitrile.
The method analyzes normetanephrine, metanephrine, 3-methoxytyramine, and 4-O-methyldopamine.