This document describes the USP analysis of sorbitol on an Agilent Hi-Plex Pb column. Sorbitol is a sugar alcohol that is metabolized slowly by the body and is used as a sugar substitute in food and in tobacco products. It is also a common ingredient in toothpaste. Additionally, sorbitol is widely used in the pharmaceutical industry as both an excipient and an active ingredient.
Sorbitol is a sugar alcohol used in food, tobacco, and pharmaceutical applications. This document details its analysis as an excipient or active ingredient.
The analysis utilizes liquid chromatography with a refractive index detector and the Agilent Hi-Plex Pb USP L34 column maintained at 50 °C.
The analysis requires a flow rate of 0.7 mL/min.
The column temperature must be maintained at 50 °C.
The relative retention time must be about 0.6 for mannitol and 1.0 for sorbitol.