This application note introduces a method for detecting artificial sweeteners in wastewater, which uses solid-phase extraction (SPE) to effectively extract sweeteners from water for LC/MS detection.
This document details an analytical method for detecting four synthetic sweeteners—acesulfame, saccharin, cyclamate, and sucralose—in waste water. The method utilizes Agilent Bond Elut Plexa solid-phase extraction (SPE) cartridges to pre-concentrate the analytes from water samples prior to detection.
| SPE Cartridge Capacity | 200 mg |
|---|---|
| SPE Cartridge Volume | 6 mL |
| Column Dimensions | 4.6 x 50 mm |
| Column Particle Size | 1.8 pm |
| Mobile Phase A | Water, 2 mM ammonium carbonate |
| Mobile Phase B | Methanol, 2 mM ammonium carbonate |
| Flow Rate | 0.6 mL /min |
| Injection Volume (20 pL) | Recovery > 86% |
The method analyzes acesulfame, saccharin, cyclamate, and sucralose.
The concentration of acesulfame, cyclamate, saccharin, and sucralose in the water sample is 1 ppb.