This document introduces the application of Agilent Hi-Plex columns, which are mainly used for the separation of carbohydrates, alcohols, and organic acids. These columns use ion-exchange ligand-exchange columns, suitable for the analysis of compounds such as simple sugars, alcohols, oligosaccharides, and organic acids in foodstuffs. The columns use isocratic conditions with water as the eluent and temperature as the main variable for control of resolution. When samples contain organic acids, especially in the presence of neutral mono- and disaccharides, Agilent Hi-Plex H is the preferred option. The column is run using water as the eluent for the analysis of sugars and organic acids or, more commonly, dilute acid for separation.
Agilent Hi-Plex columns are ion-exchange ligand-exchange columns primarily used for the separation of carbohydrates and organic acids. The range includes columns with 4% and 8% crosslinked resins, suitable for analyzing oligosaccharides, mono-, di-, and trisaccharides, as well as other compounds like alcohols.
The document details specific applications using Agilent Hi-Plex H, Hi-Plex Ca, and Hi-Plex Ca (Duo) columns. It provides chromatographic conditions, including mobile phases, flow rates, temperatures, and detectors (RI or UV), along with peak data tables for various analytes such as citric acid, glucose, and sucrose.
| Column Dimensions (Hi-Plex H) | 7.7 x 300 mm |
|---|---|
| Column Dimensions (Hi-Plex Ca) | 7.7 x 300 mm |
| Column Dimensions (Hi-Plex Ca Duo) | 6.5 x 300 mm |
| Resin Crosslinking (Hi-Plex H) | 8% crosslinked resin |
| Resin Crosslinking (Hi-Plex Ca) | 8% crosslinked resin |
| Resin Crosslinking (Hi-Plex Ca Duo) | 8% crosslinked material |
| Flow Rate (Hi-Plex H) | 0.4 mL/min to 0.6 mL/min |
| Temperature (Hi-Plex Ca) | 85 °C |
They are ion-exchange ligand-exchange columns used predominantly for the separation of carbohydrates and organic acids.
Agilent Hi-Plex H is the preferred option for samples containing organic acids, whether or not in the presence of neutral mono- and disaccharides.
Agilent Hi-Plex Ca uses 100% DI H2O as the mobile phase.