This application note describes the USP analysis of seven sugar alcohols on an Agilent Hi-Plex Ca column, including sample preparation, chromatographic conditions, and results.
This document details the analysis of seven sugar alcohols (iso-erythritol, adonitol, arabitol, mannitol, xylitol, dulcitol, and sorbitol) using an Agilent Hi-Plex Ca column. The authors demonstrate that using pure water as the mobile phase results in poor separation, with several compounds co-eluting.
To achieve better resolution, the document recommends introducing acetonitrile into the mobile phase. Specifically, a 30:70 ratio of acetonitrile to water at a flow rate of 0.20 mL/min and a temperature of 90°C significantly improves selectivity, doubles retention time, and separates all seven compounds.
| Column | Agilent Hi-Plex Ca USP L19, 4.0 x 250 mm, 8 pm |
|---|---|
| Flow rate (Optimized) | 0.20 mL/min |
| Temperature | 90°C |
| Detector | RI |
| Injection volume | 10 uL |
Introducing acetonitrile into the mobile phase significantly affects selectivity and results in better separation.
A mobile phase of 30:70 acetonitrile:100% DI H2O is recommended.
It gives extra retention for the sugar alcohols and increases the resolution between them.