This application note describes the HPLC analysis of byproducts in fermentation liquids using an Agilent Hi-Plex H column.
This document details the use of an Agilent Hi-Plex H column for analyzing byproducts and degradation products derived from biomass fermentation, such as xylose to xylitol conversion. It presents results from two specific samples: one obtained via hydrothermal digestion of straw and another following partial evaporation and fermentation.
| Column Dimensions | 7.7 x 300 mm, 8 µm |
|---|---|
| Mobile Phase | 0.005 M H2SO4 |
| Flow Rate | 0.7 mL/min |
| Injection Volume | 20 µL |
| Temperature | 60°C |
| Pressure | 4.6 MPa (46 bar, 670 psi) |
| Detector Temperature | 55°C |
The document recommends using sulfuric acid as the mobile phase.
Yes, the column is recommended for use with samples that contain high levels of organic acids.