Agilent Technologies, Inc. Rapid Profiling of Saccharides Using HPLC with ELSD for Improved Precision Application Note
Saccharides are important compounds that lack UV chromophores, making them difficult to detect with standard UV methods. While refractive index (RI) detection is common, it often suffers from baseline instability and poor sensitivity. This document proposes using evaporative light scattering detection (ELSD) combined with the Agilent Hi-Plex Ca column for improved precision and stability.
The procedure uses 100% deionized water as the mobile phase.
The system removes 5 mL/min of water at a temperature of 30 °C.
The analysis successfully separated Stachyose, Sucrose, Glucose, Fructose, and Sorbitol.