This document describes the application of the Agilent 6550 Quadrupole Time-of-Flight (Q-TOF) Mass Spectrometer for high sensitivity peptide analysis. The Agilent iFunnel technology combines highly efficient electrospray ion generation and focusing with a hexabore capillary sampling array and dual-stage ion funnel to increase ion sampling and transmission. This innovative design allows for a 10-fold increase in sensitivity for selected molecules compared to an earlier generation Q-TOF instrument without iFunnel technology.
This document describes the high sensitivity capabilities of the Agilent 6550 Quadrupole Time-of-Flight (Q-TOF) Mass Spectrometer equipped with iFunnel technology. The technology combines efficient electrospray ion generation with a hexabore capillary sampling array and dual-stage ion funnel to increase ion transmission. The note highlights that this design allows for a 10-fold increase in sensitivity for selected molecules compared to earlier generation Q-TOF instruments lacking iFunnel technology.
The document presents experimental data comparing the Agilent 6550 iFunnel O-TOF system against an Agilent 6540 O-TOF system. Results show significant increases in signal height for a synthetic peptide in both full scan MS and targeted MS/MS modes. Additionally, the note details the system's performance in protein identification workflows and peptide quantitation, demonstrating the ability to identify proteins at the 10 amol on-column level and achieve linearity over four orders of dynamic range.
| Sensitivity increase over earlier generation O-TOF | 10-fold increase |
|---|---|
| Sensitivity increase in targeted MS/MS mode | 7x increase |
| Protein identification limit | 10 amol on-column |
| Peptide quantitation limit of detection | 10 amol on-column |
| Dynamic range for quantitation | 4 orders |
It enables a 10-fold increase in sensitivity for selected molecules.
The limit of detection is 10 amol on-column for the target peptide in the presence of 150 ng of digested yeast lysate.
Yes, the system permits high sensitivity qualitative and quantitative analysis to be performed on a single system.