Agilent 6490 Triple Quadrupole LC/MS tMRM Application Note

This document introduces the Triggered MRM Acquisition method, which can simultaneously quantify and confirm target proteins.


Agilent 6490 Triple Quadrupole LC/MS tMRM Application Note - cover page
Brand
Agilent
Category
Other
Document type
Application Note
Language
English
Pages
4
File format
PDF
File size
245 KB
Published
11 June, 2012
Updated
30 September, 2023
MD5 checksum
5FBF5C35DA5FD80744348B95A67C4C2C

About this manual

This document explains the tMRM acquisition mode available on Agilent Triple Quadrupole LC/MS systems. This mode combines dynamic MRM for quantitative analysis with the generation of MRM-based product ion spectra for library identification and confirmation. It allows for quantitative and qualitative analysis on a single instrument in a single run, decreasing analysis time and increasing throughput.

What's inside

  • Introduction
  • Using the tMRM Method
  • Results and Discussion
  • Conclusions

Specifications

Max MRM transitions per peptide10
System TypeTriple Quadrupole LC/MS

Frequently asked questions

What is the benefit of tMRM acquisition?

It decreases analysis time, increases throughput, and allows for quantitative and qualitative analysis on a single instrument in a single run.

How many MRM transitions are allowed per peptide in tMRM mode?

A total of 10 MRM transitions are allowed, which can be any combination of primary and secondary types.

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